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. 2010 May 20;5(5):e10724. doi: 10.1371/journal.pone.0010724

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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(A) Expression of miRNAs from the miRNome analysis were further validated in 14 young and 14 old subject PBMCs (see Table 1B for detailed demographic data) using real-time RT-PCR as described in Materials and Methods. The histograms show normalized averages ± SEM from duplicate experiments. *P<0.05 by both Student's t-test and three-way ANOVA. (B) miR-24 and miR-221 expression was examined in 14 young and 14 old individuals using a TaqMan microarray assay as described in Materials and Methods. (C)Age-associated miRNAs that have been implicated in various cancers. See recent reviews for details [4], [35], [50].