Figure 5. Purified inter-species proteins activate the vomeronasal system and induce responses similar to native kairomones.
(A) Behavior inducing activity was found in the FPLC-A fraction and is accounted for by recombinant Mup13 (rMup-Rn13). Recombinant maltose-binding protein (rMBP) and control FPLC fractions did not initiate defensive behavior. (B) rMup-Rn13 protein exposure induces AOB activation (see quantification in Fig. S5C). n=8-12; gr, granule cell layer of the AOB; mcl, mitral cell layer of the AOB; gl, glomerular layer of the AOB; d, dorsal; m, medial; bar=100μm. **P<0.01; ***P<0.001; ANOVA followed by Tukey-Kramer HSD post-hoc analysis against PBS-soaked control gauze. Mean ± SEM. See also Fig. S5.