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. Author manuscript; available in PMC: 2011 May 14.
Published in final edited form as: Cell. 2010 May 14;141(4):692–703. doi: 10.1016/j.cell.2010.03.037

Figure 6. Isolation and characterization of a second purified kairomone.

Figure 6

(A) Avoidance and risk assessment behaviors induced by cat saliva kairomones is accounted for by recombinant Mup (rMup-Feld4). (B) cFos immunostaining of the anterior and posterior AOB following exposure to cat saliva and rMup-Feld4. (C) Quantification of calcium imaging to recombinant kairomones, rMup-Rn13 and rMup-Feld4, and recombinant Mup pheromones (a pool of mouse rMup3, 8, 17, 24 and 25, each of which is expressed in C57/BL/6J male urine) in dissociated VNs isolated from wild-type (black bars) or TrpC2−/− (white bars) male mice. Recombinant maltose binding protein (rMBP) is used as a control. n=6-24 expts. (D) Defensive behavior to recombinant Mup protein kairomones depend on VNO function. n=8-12; gr, granule cell layer of the AOB; mcl, mitral cell layer of the AOB; d, dorsal; m, medial; bar=100μm. *P<0.05; **P<0.01; ***P<0.001; ANOVA followed by Tukey-Kramer HSD post-hoc analysis. Mean ± SEM. Control odor is PBS-soaked gauze. See also Fig. S6.

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