Figure 4. Phagocytosis of P. brasiliensis yeasts by macrophages from male and female mice.

Macrophages treated or not with paracoccin (50 µg/well) were challenged with Pb FITC- conjugated yeasts for 4 hs. The cells were stained with Evans Blue and counted in a fluorescence microscope (100x) to determine the yeast Phagocitic index (3A). Panel B illustrates the result and allow to observe that no extracellular attachment of yeasts after 4 hs of incubation. The results on panel A represent the mean±SD from a representative experiment of three assays.