Fig. 3.

Co-transfection of siRNA and codon-optimized cDNA-containing vector restores wild-type target gene expression and cellular sensitivity to B[a]P exposure. MCF-7 cells were electroporated as indicated in figure key. a Quantitative real-time PCR of transfected cells for CYP1A1 mRNA, normalized to GAPDH mRNA. b Inverted microscope images showing MCF-7 viability following B[a]P exposure. c Quantification of cell viability following 96 h exposure to control or B[a]P-containing media. Cell counts are normalized to the average of cells plated at t 0