Table 1.

Primer sequences of the tested housekeeping genes ACTB, GAPDH, RPL13A and of the osteogenic markers collagen type I (CI), osteonectin (ON) and bone sialoprotein II (BSP)

Gene	Forward primer	Reverse primer	Product (bp)
Housekeeping genes
ACTBa	CTGGAACGGTGAAGGTGACA	AAGGGACTTCCTGTAACAATGCA	140
GAPDH	CGACCACTTTGTCAAGCTCA	GAGGGTCTCTCTCTTCCTCT	150
RPL13A	CTATGACCAATAGGAAGAGCAACC	GCAGAGTATATGACCAGGTGGAA	121
Osteogenic markers
CI	TTCGGAGGAGAGTCAGGAAG	CACAAGGAACAGAACAGAACAGTC	116
ON	TCCACAGTACCGGATTCTCTCT	TCTATGTTAGCACCTTGTCTCCAG	107
BSP	GCAGTAGTGACTCATCCGAAGAA	GCCTCAGAGTCTTCATCTTCATTC	121

^aPrimer sequence described by Vandesompele et al. (2002)