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. 2010 May 20;5(5):e10737. doi: 10.1371/journal.pone.0010737

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Rogalewski et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) The N-terminally 3xFLAG-tagged rat Sema6A was cloned behind the CMV/chicken beta actin promoter. (B) PCR for semaphorin-FLAG mRNA in the brain 35 days post injection of the virus. Shown is an agarose gel electrophoresis for amplimers specific for virus-originated Sema6a mRNA 35 days post injection in brain hemispheres. For the detection of AAV-3x FLAG-Semaphorin 6A nested PCR primers were used giving a final PCR product of 223 bp. The marker is a 100 bp-ladder. lane 1: (animal #22; Sema6A AAV2); lane 2: (animal #24; Sema6A AAV2); lane 3: (animal #8; empty AAV2); lane 4: (animal #11; empty AAV2); lane 5: positive control (AAV-3x FLAG-Semaphorin 6A plasmid); lane 6: negative control (water).