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. 2010 May 21;5(5):e10758. doi: 10.1371/journal.pone.0010758

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Bányai et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Recombinant protein isolated by Ni affinity chromatography (seeFig. 2.) was chromatographed on a AKTA purifier 10 System using a Superdex gel filtration column (HiLoad 10/300 Superdex 200 GL) and fractions containing pure Agrin-Nterm (horizontal bar) were pooled. Lane a of the insert shows SDS-PAGE of Agrin-Nterm of the pooled factions of AKTA chromatography, lane b indicates the pattern of the Low Molecular Weight Calibration Kit (Amersham Pharmacia Biotech, Uppsala, Sweden; Mr values: 14,400, 20,100, 30,000, 45,000, 66,000, and 97,000).