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. 2010 May 21;5(5):e10765. doi: 10.1371/journal.pone.0010765

Table 2. Minimal turnover numbers and second-order rate constants of the LZ-catalyzed RNA18 degradationa.

peptide RNA18 cleavage time V obs k obs (≤k cat) k 2obs (≤k 2)
µM µM % h nM min−1 min−1 M −1 min−1
GCN4 LZ35 30 50 17 2 70.8 0.0024 47
cJun LZ36 60 170 28 4 198 0.0033 19
cJun 20 90 50 48 15.6 0.0008 9

aBased on the degradation rates of full-length 18-mer RNA substrate in 20 mM Tris-HCl, 80 mM KCl, pH 7.2, 37°C.