Table 2. Minimal turnover numbers and second-order rate constants of the LZ-catalyzed RNA18 degradationa.
peptide | RNA18 | cleavage | time | V obs | k obs (≤k cat) | k 2obs (≤k 2) | |
µM | µM | % | h | nM min−1 | min−1 | M −1 min−1 | |
GCN4 LZ35 | 30 | 50 | 17 | 2 | 70.8 | 0.0024 | 47 |
cJun LZ36 | 60 | 170 | 28 | 4 | 198 | 0.0033 | 19 |
cJun | 20 | 90 | 50 | 48 | 15.6 | 0.0008 | 9 |
aBased on the degradation rates of full-length 18-mer RNA substrate in 20 mM Tris-HCl, 80 mM KCl, pH 7.2, 37°C.