Table 2. ATF2 transcriptional targets.
| Gene | Context | Methods | Refs |
|---|---|---|---|
| Extracellular stimuli | |||
| IL8 | ATF2 recruits macroH2A nucleosome to repress IL8 transcription | HeLa and Namalwa B-cells, WB and Luc | 72 |
| Pdgfra | ATF2-null mice exhibit decreased PDGFRα expression levels that are rescued on re-expression of ATF2 | Mutant mice and CHO cells, WB and Luc | 73 |
| MMP2 | ATF2 activates MMP2 transcription | MCF10A cells and Luc | 78 |
| PLAU | ATF2 cooperatively induces PLAU in response to IL-1 and TPA | HepG2 cells, WB and Luc | 216 |
| Crip2 | TGFβ upregulation of CRP2 requires ATF2 | Rat and mouse vascular smooth muscle cells, WB and Luc | 217 |
| Psen1 | Presenilin 1 expression is reduced in Atf2-null keratinocytes | Murine keratinocytes and WB | 115 |
| SELE | ATF2 contributes to E-selectin induction during inflammatory response | Human endothelial cells, WB and Luc | 218 |
| TNF | Autoamplification of death signals are mediated by ATF2 | Jurkat and CEM cells, and WB | 219 |
| ATF3 | Radiation-activated ATM signalling induces ATF3 through ATF2 | Normal human diploid fibroblasts and WB | 220 |
| Nos2a | ATF2 mediates NOS2A upregulation in rat glial cells | C6 glial cells and Luc | 221 |
| IFNG | Jun–ATF2 dimers regulate IFNG transcription through proximal promoter | Jurkat cells, WB and Luc | 222 |
| Hspa5 | ATF2 and CREB mediate stress induction of HSPA5 | 9L rat brain tumour cells and WB | 223 |
| Th | ATF2 positively regulates tyrosine hydroxylase transcription | PC12D cells, WB and Luc | 224 |
| Cell cycle and transcription | |||
| CCND1 | p38 and ATF2 induce cyclin D1 in breast cancer in response to oestradiol and spermine | MCF7 cells, WB and Luc | 119 |
| Ccna1 | ATF2 mediates serum induction of cyclin A | Rat chondrosarcoma cells, WB and Luc | 114 |
| Rb1 | ATF2 modulates RB during skeletal growth | ATDC cells and primary chondrocytes, WB and Luc | 112 |
| PI5 | ATF2 binds directly to the PI5 promoter (which encodes maspin) | Mutant mice, MEFs, MCF7 cells, WB and Luc | 116 |
| GADD45A | ATF2 is recruited by OCT1 and NF1 to the GADD45A promoter to activate transcription | Mutant mice, MEFs, MCF7 cells, WB and Luc | 116 |
| PKC | PKC phosphorylates ATF2 to activate JUN transcription | F9 teratoma, MEFs, HeLa cells, WB and Luc | 90 |
| Ppargc1a | ATF2 mediates exercise-induced PGC1α upregulation | C2C12 myocytes, WB and Luc | 225 |
| Atf and Creb, Jun and Fos family genes | ATF2 transcriptionally regulates other members of the Atf and Creb, and Jun and Fos families, contingent on cell type and stimulus context | F9 teratoma, COS-1, MCF7 cells, WB and Luc | 4,82, 226 |
| Mapk14 | ATF2 negatively feeds back on p38 signalling through transcriptional induction of MAPK phosphatases | Mutant mouse tissue and cells, and WB | 68 |
| Cell death | |||
| Bcl2 | ATF2 controls Bcl2 promoter activation in chondrocytes | Mutant mice, WB and Luc | 94 |
| TRAIL | Auto-amplification of death signals are mediated by ATF2 | Jurkat and CEM cells, and WB | 219 |
| ACHE | ATF2 mediates hydrogen peroxide-induction of acetylcholinesterase | 293T cells, WB and Luc | 227 |
ACHE, acetylcholinesterase; ATM, ataxia-telangectasia mutated; CCND1, cyclin D1; CHO, Chinese hamster ovary; Crip2, cysteine-rich intestinal protein 2; Hspa5, heat shock protein A5; IFNG, interferon (IFN)-γ; IL8, interleukin-8; Luc, luciferase assay; MEF, mouse embryonic fibroblast; MMP2, matrix metalloproteinase 2; Nos2a, nitric oxide synthase 2A; Pdgfra, platelet derived growth factor A; PI5, protease inhibitor 5; PLAU, urinary palminogen activator; Ppargc1a, peroxisome proliferator-activated receptor-γ coactivator 1α; Psen1, presenilin 1; SELE, E selectin; TGFβ, transforming growth factor-β; Th, tyrosine hydroxylase; TNF, tumour necrosis factor; TRAIL, tumour necrosis factor-related apoptosis-inducing ligand; WB, western blot.