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. Author manuscript; available in PMC: 2011 May 26.
Published in final edited form as: J Am Chem Soc. 2010 May 26;132(20):6929–6931. doi: 10.1021/ja102103n

Fig. 2.

Fig. 2

NMR relaxation measurements on bicelles and 15N-labeled subtilosin A for the evaluation of paramagnetic doping effect: (A) with 2.56 mM copper-chelated lipid; (B) with 30 mM copper-EDTA. (C) Difference in spin-lattice relaxation rates with (R1) and without (R1’) copper-chelated lipid; labels I and II correspond to 15N signal from the rigid transmembrane (78.3 ppm) and flexible membrane-surface (112.6 ppm) regions respectively. (D) Difference in signal intensities obtained from bicelles with and without copper-chelated lipid. (E) Data showing the RF-induced heating on bicelles measured as explained in Fig. S8. (F-H) A comparison of 13C CP signal intensities measured from bicelles with no copper (E), 30 mM Cu-EDTA (blue) and 2.5 mM Cu DMPE-DTPA (red).