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. Author manuscript; available in PMC: 2011 May 19.
Published in final edited form as: Bioconjug Chem. 2010 May 19;21(5):903–910. doi: 10.1021/bc900461h

Figure 6.

Figure 6

Effects of the OHT-6C-BODIPY conjugate on ER alpha stability. MCF7 cells were seeded in hormone-free medium for three days, then treated with different compounds for 24 hours. Lane 1: DMSO; Lane 2: 10 nM estradiol; Lane 3: 100 nM fulvestrant; Lane 4: 100 nM 4-hydroxytamoxifen; Lane 5: 1 μM OHT-6C; Lane 6: 10 μM OHT-6C-BODIPY. ER alpha levels in whole cell extracts were determined by immunoblotting with anti-ERα antibody. GAPDH was used as the loading control. Relative ER alpha levels (versus untreated cells) are shown in the corresponding histogram