Figure 2.
Matrix morphology of PCL/ECM (PE) constructs generated in flow perfusion culture of increasing durations (4, 8, 12, and 16 days) for PE4, PE8, PE12, and PE16 constructs. Histological sections stained with hematoxylin and eosin to visualize the distribution of cells and extracellular matrix proteins are shown in (A) with the scale bar representing 100 µm. X-ray images depicting radiopaque regions of mineralized matrix are shown in (B) with the scale bar representing 1 mm. Scanning electron micrographs of the top surface illustrating surface characteristics are shown in (C) with arrows indicating mineral nodules and the scale bar representing 100 µm.