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Stability of the 5′–3′ complexes. The 3′ and 5′ transcripts were heated, followed by cooling to room temperature, to allow 5′–3′ complex formation. Subsequently, the samples were diluted and aliquots were incubated for 10 min at 37, 50, 55, 60, or 65°C. The melting temperature was calculated by PhosphorImager quantification of the gels. The positions of the radiolabeled 3′ transcript and the 5′–3′ complex are indicated.
