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FAK phosphorylation is regulated by inhibitors of MLC phosphorylation. Cells were cultured on micropatterned substrates for 16 hours and treated with inhibitors for 30 minutes. A, Western blotting for pFAK[Y397] and total FAK. Relative amounts of (B) pFAK[Y397] and (C) total FAK. The results represent two independent experiments with mean ± standard deviation (n=3). *p<0.05 relative to no treatment controls that received fresh media without inhibitors.
