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. Author manuscript; available in PMC: 2011 Apr 1.
Published in final edited form as: J Infect Dis. 2010 Apr 1;201(7):1007–1015. doi: 10.1086/651170

Figure 1. Cell surface sialic acid level after DAS181 treatment of human respiratory epithelial cells.

Figure 1

Monolayers of human epithelial A549 cells were treated with various doses of DAS181 as indicated for 30 min at 37°C. After incubation with DAS181, the cells were fixed and assayed for the surface level of sialic acids using SNA and MAL-II (A). Error bars represent one standard deviation above and below the mean of three replicate samples. After washing, the cells were incubated with S. pneumoniae (strain D39) at the indicated input amount for 1 hr at 37 °C. After washing, the adherent bacteria were quantitated by viable counts (B). Solid lines: bacterial adhesion to the cells treated with medium alone; dashed lines: bacterial adhesion to the cells treated with DAS181. Student’s t-test was used to determine P values; *=p<0.05.