Fig. 6. CM6 inhibits MCF10A cell spreading on the LG2 module of rat Ln-332.

(A) 96-well microplates were coated with Ln-332 (1 μg/ml), GST (50 μg/ml), rLG2 (50 μg/ml), rLG3 (50 μg/ml) at 4°C overnight. MCF10A cells were added to wells (5000/well) and incubated for 5 h at 37°C in a humidified, 5% CO₂, 95% air atmosphere. After incubation, attached cells were fixed and stained with Hoechst (blue) and phalloidin (green) in PBS containing 3% BSA for 2 h at RT. Cell spreading was quantified by manually counting spread cells in five representative microscope fields. (B) CM6 significantly reduced cell spreading on Ln-332 (N=5; p=0.008) and rLG2 (N=5; p=0.008). In contrast, CM6 had little effect on cell spreading on rLG3 module (N=5; p=0.222) or GST (N=5; p=0.095).