TABLE 3.
TANDEM MASS SPECTROMETRIC ANALYSIS OF DIFFERENTIALLY EXPRESSED PROTEIN SPOTS
| Original SSP | ID No. | P Value | Experimental kDa | Experimental pI | Mean Spot Volume, HS | Mean Spot Volume, COPD Stage 2 | Mean Fold Change | Protein ID |
|---|---|---|---|---|---|---|---|---|
| 2304 | 1 | — | 26.6 | 4.8 | — | — | −7* | Apolipoprotein A1 (5) |
| 1019 | 2 | — | — | — | — | — | +9* | Not detected |
| 7203 | 3 | — | — | — | — | — | +9* | Not detected |
| 6606 | 4 | 0.002 | 46.8 | 5.5 | 1,085.8 | 2,469.9 | 2.3 | Hypothetical protein (1) |
| 5201 | 5 | 0.002 | 25.2 | 5.4 | 9,076.8 | 4,386.1 | 0.5 | Albumin (4) |
| 3107 | 6 | 0.004 | 16.7 | 4.9 | 11,974.6 | 17,568.2 | 1.5 | PSP94 (2) |
| 6317 | 7 | 0.004 | 26.8 | 5.6 | 8,872.1 | 5,009.3 | 0.6 | Albumin (8) |
| 2308 | 8 | 0.006 | 26.3 | 4.9 | 2,301.3 | 498.6 | 0.2 | Apolipoprotein A1 (11) |
| 6002 | 9 | 0.006 | 13.0 | 5.5 | 964.3 | 1,935.3 | 2.0 | Apolipoprotein A1 (4) |
| 7112 | 10 | 0.006 | 15.7 | 5.9 | 24,410.6 | 12,301.0 | 0.5 | Albumin (6) |
| 3713 | 11 | 0.006 | 49.3 | 5.0 | 757.1 | 381.2 | 0.5 | Albumin (12) |
| 2709 | 12 | 0.007 | 54.5 | 4.8 | 796.3 | 428.9 | 0.5 | Albumin (3) |
| 8721 | 13 | 0.008 | — | — | 2,432.0 | 1,204.8 | 0.5 | Not detected |
| 1122 | 14 | 0.013 | 18.1 | 4.7 | 5,176.9 | 2,891.5 | 0.6 | Lipocalin-1 (3) |
| 2506 | 15 | 0.013 | 35.6 | 4.7 | 453.3 | 1,284.3 | 2.8 | Albumin (3) |
| 4626 | 16 | 0.013 | 47.3 | 5.4 | 1,178.9 | 2,154.3 | 1.8 | Lactoferrin (6) |
| 3220 | 17 | 0.014 | 23.7 | 5.1 | 698.8 | 430.3 | 0.6 | Apolipoprotein A1 (1) |
| 302 | 18 | 0.015 | 27.4 | 0 | 10,628.1 | 15,246.1 | 1.4 | IgJ (2)PLUNC (1) |
| 2009 | 19 | 0.017 | 12.5 | 4.8 | 1,136.9 | 514.1 | 0.5 | Albumin (2) |
| 3013 | 20 | 0.017 | 13.2 | 5.0 | 2,354.6 | 1,536.6 | 0.7 | Albumin (2) |
| 4414 | 21 | 0.017 | 30.2 | 5.3 | 2,253.6 | 1,195.0 | 0.5 | Zinc-α2-glycoprotein (2) |
| 1606 | 22 | 0.02 | 45.5 | 0 | 1,587.5 | 2,785.3 | 1.8 | Albumin (4) |
| 6619 | 23 | 0.022 | 46.5 | 5.7 | 670.4 | 1,356.1 | 2.0 | Albumin (7) |
| 4202 | 24 | 0.023 | 20.5 | 5.1 | 1,126.6 | 514.8 | 0.5 | α2-HS glycoprotein (2)Transferrin (2)α1-Antitrypsin (4)Albumin (16) |
| 3607 | 25 | 0.026 | 47.7 | 4.9 | 1,530.9 | 2,553.0 | 1.7 | α2-HS glycoprotein (2)Hemoglobin (1) |
| 4002 | 26 | 0.026 | — | — | 3,642.9 | 6,634.8 | 1.8 | Not detected |
| 7104 | 27 | 0.026 | 16.7 | 5.8 | 4,960.4 | 1,830.9 | 0.4 | Lactoferrin (4)Albumin (2) |
| 5905 | 28 | 0.028 | 75.0 | 5.5 | 1,359.8 | 2,653.9 | 1.9 | Albumin (7) |
| 4418 | 29 | 0.029 | 31.8 | 5.4 | 2,512.3 | 1,587.5 | 0.6 | Albumin (4) |
| 4625 | 30 | 0.029 | 44.5 | 5.4 | 4,071.2 | 2,420.9 | 0.6 | Transthyretin (3)IgA (1) |
| 4101 | 31 | 0.029 | 17.1 | 5.1 | 793.6 | 411.3 | 0.5 | Transthyretin (3)IgA (1) |
| 4623 | 32 | 0.031 | 46.7 | 5.3 | 2,710.0 | 4,431.2 | 1.6 | Albumin (3)Unnamed protein (2)Lactoferrin (1) |
| 4713 | 33 | 0.033 | 49.9 | 5.3 | 5,027.5 | 2,735.4 | 0.5 | Albumin (9) |
| 4104 | 34 | 0.035 | — | — | 329.3 | 562.5 | 1.7 | Not detected |
| 8008 | 35 | 0.038 | 12.6 | 0 | 1,971.7 | 2,566.3 | 1.3 | SAP (1) |
| 3206 | 36 | 0.042 | 21.5 | 4.9 | 7,407.0 | 4,276.0 | 0.6 | Albumin (3) |
| 3208 | 37 | 0.042 | 23.5 | 4.9 | 12,879.1 | 6,916.0 | 0.5 | Albumin (4) |
| 3212 | 38 | 0.043 | — | — | 630.6 | 290.7 | 0.5 | Not detected |
| 2521 | 39 | 0.049 | — | — | 339.9 | 608.6 | 1.8 | Not detected |
| 2111 | 40 | 0.05 | 18.0 | 4.8 | 10,164.1 | 5,889.4 | 0.6 | Lipocalin-1 (4) |
Definition of abbreviations: α2-HS glycoprotein = α2-Heremans–Schmid glycoprotein; 2-DGE = two-dimensional gel electrophoresis; COPD = chronic obstructive pulmonary disease; HS = healthy smokers; pI = isoelectric point; PLUNC = palate, lung, and nasal epithelium clone protein; PSP94 = prostate secretory protein of 94 amino acids; SAP = salivary acidic protein; SSP = sample spot protein.
Protein spots determined as significant (P ≤ 0.05) from comparison of healthy smokers and smokers with stage 2 COPD.
Sputum 2-DGE spot profiles were excised from gels and subjected to tandem mass spectrometry to determine protein identifications. Values in parentheses denote the number of peptides detected per protein.
For qualitatively detected protein spots, spot differences are expressed as the difference in the number of gels containing that protein spot between the two groups, according to the principle stated in Methods.