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Pressure-volume curves and lung histology. (A) The deflation limbs of pressure-volume curves were not different between the groups. (B, C) Lung histology assessed after staining with hematoxylin and eosin was similar for both groups. Histology was typical of immature lung, including thickened alveolar septal walls and patchy atelectasis. More alveolar fluid was observed in control lambs than in lambs treated with recombinant human surfactant protein D (+rhSP-D). Scale bar: 100 μm.
