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. Author manuscript; available in PMC: 2010 May 21.
Published in final edited form as: Exp Mol Pathol. 2008 Jan 11;84(2):102–112. doi: 10.1016/j.yexmp.2007.12.003

Fig 6.

Fig 6

Tissue culture showing MDB formation in vitro using primary cultures from 1 month DDC withdrawn (wd) mice n=9 CK8/UB double immunostain (x800). A) Cell suspension of DDC Wd mouse. B) 6 day primary culture from a DDC Wd mouse where numerous MDBs (arrows) had spontaneously formed in large multinucleated hepatocytes. C) DDC Wd hepatocytes with DMSO vehicle added where MDBs formed. D) DDC Wd hepatocytes where TSA was added. No MDBs were formed. E) DDC Wd hepatocytes where Aza was added, MDBs formed (arrow). F) DDC Wd hepatocytes with both TSA and Aza were added, residual tiny MDBs had persisted in the middle hepatocyte (x800).