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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Int J Cancer. 2010 Jun 1;126(11):2520–2533. doi: 10.1002/ijc.24988

Figure 3.

Figure 3

Effect of green tea polyphenols (GTP) on reversal of methylation in human prostate cancer LNCaP cells. A, Map of the GSTP1 gene and putative CpG islands. The relative position and sizes of the 7 exons (solid bars) of the GSTP1 are depicted. B, MS-PCR for GSTP1 promoter on genomic DNA isolated from cells after treatment with 10μg/mL GTP for 7 days and 10μM 5-Aza-dC for 3 days. Displayed are the products generated with primers specific for unmethylated GSTP1 CpG island alleles (U) and for hypermethylated GSTP1 CpG island alleles (M). C, methylation analysis of individual clones from proximal and D, distal GSTP1 promoter after GTP and 5-Aza-dC treatment. Reduction in GSTP1 CpG island hypermethylation was noted in LNCaP DNA by these treatments. At least 15 PCR clones from each treatment were sequenced; the CpG island DNA methylation pattern for selected clones are displayed. Closed circles indicate a methylated CpG and open circles indicate an unmethylated CpG. The details are described in ‘Materials and Methods’ section.