Figure 5.

Chymotrypsin-like proteasome activity in the hypothalamus and cortex of 7- to 9-mo-old WT and pcd mice. Activities of soluble and particulate fractions from the hypothalamus (left) and cortex (right) of WT and mutant animals were measured by detecting the fluorescence of free AMC released after the hydrolysis of fluorogenic substrate Suc-LLVY-AMC (excitation 380 nm, emission 460 nm). There was no statistical difference between the level of proteasome activity in WT vs. pcd mice for either the soluble or particulate fractions of the hypothalamus or cortex.