Figure 1.
Structures and in vitro biophysical properties. A) Molecular structure of C12K-β12 and C12(ω7)K-β12 (MW 781 g/mol); difference is highlighted by dashed circles. B) Dose-dependent self-assembly in PBS after 2 h incubation at room temperature. CAC was evaluated by extrapolating the curve to the intercept with the x axis. C) Hemoglobin leakage from human erythrocytes (10% hematocrit) in PBS as determined after 15 min incubation under shaking at 37°C and compared with 0.2% Triton X-100. D) XTT cytotoxicity assay using primary fibroblasts after 24 h culture. Solid circles, C12K-β12; open circles, C12(ω7)K-β12. Error bars = sd calculated from ≥2 independent experiments performed in duplicate. Lack of error bars indicates consistency.