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. 2010 Feb 10;82(6):1076–1087. doi: 10.1095/biolreprod.109.082867

FIG. 2.

FIG. 2.

A) PRL and IGFBP1 mRNA levels in MSC treated with 1 mM 8-Br-cAMP for 14 days expressed as fold-change compared to 14 days, vehicle control. Time course of PRL and IGFBP1 protein expression in conditioned media from MSC treated with 1 mM 8-Br-cAMP were normalized to total RNA levels (n = 3). B) Expression of PRL, IGFBP1, and STC1 mRNA and/or protein in HDFn (human dermal fibroblasts, neonatal) treated with 1 mM cAMP for 14 days. PRL, IGFBP1, and STC1 mRNA levels are expressed as fold-change to time control (vehicle control at 3, 7, 14, and 18 days) normalized to t = 0. Note the difference in scale between A and B. PRL protein secretion at different culture time points was normalized to total RNA. No IGFBP1 secretion by HDFn occurred with or without cAMP treatment. Asterisk indicates statistically significant difference compared to vehicle control at each time point (P ≤ 0.05). Error bars represent the SEM.