Skip to main content
. 2010 Feb 24;82(6):1103–1111. doi: 10.1095/biolreprod.109.083097

FIG. 4.

FIG. 4.

Effect of reducing Pou5f1 expression on SSC maintenance in vitro. A) Relative Pou5f1 gene expression in THY1+ germ cell cultures at 18 h after treatment with nontargeting control or Pou5f1 siRNA. Expression of Pou5f1 transcript was reduced by 77% ± 3% in Pou5f1 siRNA-treated cultures compared with those treated with nontargeting control siRNA (n = 3 different cultures). B) Western blot analysis of POU5F1 protein expression at 18 h after treatment with nontargeting control or Pou5f1 siRNA. C) Effect of Pou5f1 siRNA treatment on total germ cell maintenance in cultures of THY1+ spermatogonia at 7 days after transfection. D) Effect of Pou5f1 siRNA treatment on maintenance of SSCs at 7 days after transfection using functional transplantation as an assay. No significant difference was found between control and Pou5f1 siRNA treatments for either total germ cell numbers or SSC numbers. Data are the mean ± SEM for three different replicate cultures, and the asterisk denotes significant difference at P < 0.05.