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. 2010 Feb 24;82(6):1103–1111. doi: 10.1095/biolreprod.109.083097

FIG. 5.

FIG. 5.

Impact of impairing Pou3f1 gene expression on survival of THY1+ germ cells in vitro. A) Effect of Pou3f1 siRNA treatment (30 h) on the percentage of apoptotic cells. The asterisk denotes significant difference between means of treatments. B) Examination of GDNF-regulated Pou3f1 expression through the PIK3/AKT signaling pathway using quantitative real-time PCR analysis. THY1+ germ cells containing SSCs were cultured in the presence of GDNF (+GDNF) and subjected to 18-h withdrawal of the growth factor (−GDNF), followed by replacement of GDNF for 4 h (+GDNF) in the presence of vehicle (dimethyl sulfoxide), the PIK3-specific inhibitor LY294002 (+LY [10 μM]), or AKT inhibitor IV (+AKT Inh. [20 nM]). Data are the mean ± SEM for three different replicate cultures, and bars with different letters are significantly different at P < 0.05. The inhibitors prevented the GDNF rebound at 4 h after an 18-h deprivation.