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. 2010 Feb 17;82(6):1112–1118. doi: 10.1095/biolreprod.109.081844

FIG. 3.

FIG. 3.

Association of TAZ and PPARG with the PPRE-containing site of the human IGFBP1 in vivo. A) PPARG mRNA in HuF cells detected by RT-PCR in total RNA. B) PPARG protein detected in the nuclear (n) fraction of HuF cells by Western blot with specific antibody. PPARG was not detected in the cytosolic extract (c). C) Chromatin was extracted from HuF cells. ChIP experiments were performed with anti-TAZ and anti-PPARG antibodies. An association of TAZ and PPARG with the PPRE-containing genomic region (−1197 to −1002 bp from the TSS) of the human IGFBP1 gene was detected. The control region of the IGFBP1 gene (−8869 to −8377 bp from the TSS), which contains no PPRE, did not show this association. The PCR on the input chromatin template (Input, 1:3100) served as a positive control and that of nonspecific rabbit IgG-precipitated template as a specificity control. Ab, antibody; negative c., negative control.