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Integrity of the BTB is impaired in Epas1Δ/Δ testes. The functional integrity assay displayed increased diffusion of FITC into the seminiferous tubules of Epas1Δ/Δ testes (arrow). In direct contrast, FITC staining is confined to the basal region of the seminiferous epithelium of control testes. Original magnification ×400.
