Table 1.
Kd and kpol for incorporation and misincorporation by wild-type (WT) and ΔTL RNA polymerase (RNAP)
| RNAP | WT | ΔTL | ||
|---|---|---|---|---|
| kpol (s-1) | Kd (μM) | kpol (s-1) | Kd (μM) | |
| cGTP | 100 | 20 | 1.6 ± 0.1 × 10-3 | 36 ± 6 |
| ncGTP | 2.7 ± 0.3 × 10-2 | 2800 ± 600 | 4.2 ± 0.5 × 10-4 | 840 ± 190 |
| c2'dATP | 5.5 ± 0.2 × 10-2 | 400 ± 30 | 1.3 ± 0.1 × 10-3 | 50 ± 9 |
| c3'dATP | 1.4 ± 0.1× 10-1 | 55 ± 8 | 2.7 ± 0.2 × 10-3 | 57 ± 11 |
Incorporation of cGTP was studied in ECG1, misincorporation of ncGTP, c2'dATP and c3'dATP - in ECA. Kd and kpol (reaction rate at saturating nucleotide triphosphate concentration) for incorporation by WT RNAP were obtained by fitting the kinetics data into a simple kinetic model as described in Additional File 1:Supplementary Methods. The rest of the data were fitted into the Michaelis-Menten equation as described in Materials and Methods. Errors are the standard error of data fitting. The values were rounded to two significant digits.