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Discrimination against 2' and 3'-deoxy substrates by ΔTL RNA polymerase.
| cATP(ECA)/ c2'dATP(ECA) |
cCTP(ECC)/ c2'dCTP(ECC) |
cGTP(ECG3)/ c2'dGTP(ECG2) |
cUTP(ECU)/ TTP(ECU) |
cATP(ECA)/ c3'dATP(ECA) |
cGTP(ECG3)/ c3'dGTP(ECG2) |
|
|---|---|---|---|---|---|---|
| Discrimination, folds |
~6 | ~11 | ~3 | ~17 | ~3 | ~0.4 |
Kinetic discrimination was quantified as a ratio of the rate of incorporation of 1 mM cNTP to the rate of misincorporation of 1 mM of a corresponding c2'dNTP or c3'dNTP in elongation complexes identified.
