Table 5.
Contribution of individual fidelity steps.
| Fidelity contribution | Open active centre | Trigger loop folding | Total |
|---|---|---|---|
| (kpol [cGTP]/Kd [cGTP])/(kpol [ncGTP]/Kd [ncGTP]) | 87 | 5950 | 518000 |
| Kinetic kpol [cGTP]/kpol [ncGTP] | 3.8 | 970 | 3700 |
| Affinity Kd [ncGTP]/Kd [cGTP] | 23 | 6.1 | 140 |
| (kpol [cGTP]/Kd [cGTP])/(kpol [c2'dATP]/Kd [c2'dATP]) | 1.7 | 21000 | 36000 |
| Kinetic kpol [cGTP]/kpol [c2'dATP] | 1.2 | 1500 | 1800 |
| Affinity Kd [c2'dATP]/Kd [cGTP] | 1.4 | 14 | 20 |
| (kpol [cGTP]/Kd [cGTP])/(kpol [c3'dATP]/Kd [c3'dATP]) | 0.96 | 2030 | 1990 |
| Kinetic kpol [cGTP]/kpol [c3'dATP] | 0.6 | 1180 | 710 |
| Affinity Kd [c3'dATP]/Kd [cGTP] | 1.6 | 1.8 | 2.8 |
Values were quantified using data of Table 1: 'Open active centre' values were generated from the row identifier ratios using the ΔTL data; 'Total' values were generated from the row identifier ratios using the wild-type (WT) data; 'TL folding' values were generated from the row identifier ratios using the ratio of the WT ratio over the ΔTL ratio.