Figure 1.
Susceptibility of PBMC of 14 rhesus monkeys to in vitro SIVsmE660 infection. (A) CD8+ T lymphocyte-depleted PBMC were infected with SIVsmE660 and p27 production was assayed in the culture supernatant by ELISA. Susceptibility to in vitro infection is expressed as the relative TCID50 of the SIVsmE660 virus stock using the Spearman-Karber method. (B) PBMC of the same animals were assayed again for in vitro susceptibility to SIVsmE660 after a three month interval. A significant positive correlation was observed between the values obtained from PBMC of each monkey in these repeated assays. (C, D) CD8+ T lymphocyte-depleted PBMC were infected with SIVsmE660, samples collected serially and grouped for display as (C) sensitive or (D) resistant to SIV replication on the basis of their TCID50 values.