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Standard ch-1a curve of the PRRSV real-time qRT-PCR assay. A 10-fold serial dilutions ranging from 9.6 to 9.6 × 105 copies of PRRSV RNA were tested in the real-time qRT-PCR. The standard formula is y = - 3.528x + 15.60 and the correlation co-efficient is 0.999.
