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The sensitivity of real-time qRT-PCR for detection of the PRRSV. A 10-fold dilution series of total RNA extracted from a field sample ranging from 10-1 to 10-7 dilutions were tested in parallel in the qRT-PCR assay and in the conventional RT-PCR. The detection limit for the real-time qRT-PCR assay was a 10-5 dilution of sample RNA.
