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The sensitivity of conventional RT-PCR for detection of the PRRSV. The detection limit for the conventional RT-PCR assay was a 10-4 dilution of sample RNA. Lanes 1--7: 10-fold dilution series of sample total RNA ranging from 10-1 to 10-7 dilutions; lane N: negative control having no template; lane M: DL2000 DNA ladder Maker (TAKARA).
