Table 1.
Annealing temperature of Nf/Nr oligonucleotide pair optimization by means of Ct values
| Annealing temperature | Cycle threshold (Ct) |
|---|---|
| 53 | 23.12 |
| 55 | 22.90 |
| 56 | 21.26 |
| 57 | 23.78 |
| 58 | 25.62 |
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