Table 2.
Determination of optimized primers concentration for the PRRSV one step real-time RT-PCR
| primers concentration (μM) | Cycle threshold (Ct) |
|---|---|
| 0.1 | > 40 |
| 0.2 | 37.11 |
| 0.3 | 29.05 |
| 0.4 | 25.60 |
| 0.5 | 25.21 |
| 0.6 | 24.90 |
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