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Oligonucleotide primers designed for PRRSV amplification by conventional RT-PCR and one step real-time RT-PCR
| Length | |||
|---|---|---|---|
| Primer | Sequence (5'-3') | Primer (bp) | Product (bp) |
| Nf | CCCGGGTTGAAAAGCCTCGTGT | 22 | 228a |
| Nr | GGCTTCTCCGGGTTTTTCTTCCTA | 24 | |
| 371f | CCCGGGTTGAAAAGCCTCGTGT | 22 | 371b |
| 371r | TGTAACTTATCCTCCCTGAATCTG | 24 | |
a Length of product amplified by one step real-time RT-PCR primer pair.
bLength of product amplified by conventional RT-PCR primer pair.
