Table 1.
Passage history and titer (in C6/36 cells) of the 12 dengue virus strains used in this study
| Serotype | Strain ID | Country of isolation | Source | Collection Year | Passage History1 |
Titer (log10 pfu/ml) |
Obtained from2 |
|---|---|---|---|---|---|---|---|
| DENV-1 | JKT 85-1415 | Indonesia | Human serum | 1985 | C6/36 p2 | 7.2 | WRCEVA |
| DENV-1 | 1335 TVP | Sri Lanka | Human serum | 1981 | Inoculated mosquito-1X, C6/36 p2 | 7.2 | WRCEVA |
| DENV-1 | AusHT15 | Australia | Human serum | 1983 | C6/36 p2 | 7.5 | WRCEVA |
| DENV-2 | Tonga/1974 | Tonga | Human serum | 1974 | Mosquito-1X, C6/36 p5 | 8.0 | NIAID |
| DENV-2 | DOO-0372 | Thailand | Human serum | 1988 | Previous history unknown, C6/36 p8 | 8.0 | NIAID |
| DENV-2 | NGC Proto | New Guinea | Human serum | 1944 | Inoculated monkey- 1X | 7.5 | NIAID |
| DENV-3 | 89 SriLan 1: D2783 |
Sri Lanka | Human serum | 1989 | C6/36 p2 | 7.6 | UNC |
| DENV-3 | 89 SriLan 2: D1306 | Sri Lanka | Human serum | 1983 | C6/36 p2 | 7.6 | UNC |
| DENV-3 | Sleman/78 | Indonesia (Java) | Human serum | 1978 | Mosquito-1X, Vero p2, C6/36 p4 | 7.2 | NIAID |
| DENV-4 | 1228 TVP | Indonesia | Human serum | 1978 | Mosquito p2, C6/36 p2 | 7.1 | WRCEVA |
| DENV-4 | 779157 | Taiwan | Human serum | 1988 | C6/36 p5 | 7.4 | WRCEVA |
| DENV-4 | BeH 403714 | Brazil | Human serum | 1982 | C6/36 p3 | 7.2 | WRCEVA |
1cell type for passage followed by total number of passages (p) in that cell type
2 WRCEVA: provided by Dr. Robert Tesh at the World Reference Center of Emerging Viruses and Arboviruses at the University of Texas at Galveston (UTMB); NIAID: provided by Dr. Stephen Whitehead, NIAID, NIH; UNC: provided by Dr. Aravinda de Silva, Department of Microbiology and Immunology, University of North Carolina.