Fig. 2. cPLA₂ inhibitor AACOCF₃ attenuates tubule formation and migration in irradiated vascular endothelial cells.

Tubule formation in A) 3B11 cells or B) primary MPMEC. Cells were cultured onto Matrigel in the absence (control) or presence of 1 μM AACOCF₃ for 30 minutes and then irradiated with 3 Gy. A) Representative micrographs of capillary tubule formation taken 5 hours after treatment are shown. Tubule formation was quantified as the number of tubule branches per high power field (4 HPF per sample). Shown are bar graphs of the average tubule formation for 3B11 (A) and MPMEC (B) with SEM from three independent experiments; *, p < 0.05. Migration assay is shown for C) 3B11 cells or D) MPMEC cells were added to the top chamber of 24 well plates with 8 μm matrigel-coated inserts. Fresh medium was added to the bottom chamber, and both chambers were treated with vehicle (control) or 1 μM AACOCF₃ for 30 minutes prior to irradiation with 3 Gy. After 24 hours, the insert chambers were stained with DAPI and migrated cells were counted (6 HPF per sample). Shown are representative micrographs of migrated 3B11 taken 24 hours after treatment (C) and bar graphs of the average number of migrated cells per HPF for 3B11 (C) and MPMEC (D) with SEM from three independent experiments; *, p < 0.05.