Figure 3.
Effects of osmolytes on MBP-OLF stability. a) MBP-OLF(wt). b) MBP-OLF(D380A). c) MBP-OLF(I477N). d) MBP-OLF(I477S). e) MBP-OLF(K423E). For b)-e), MBP-OLF mutant (no osmolyte) appears as the leftmost melting curve using the same scheme as in Fig 2d, and MBP-OLF(wt) appears as a grey. f) Color key to osmolytes.
