. Author manuscript; available in PMC: 2011 May 21.

Published in final edited form as: ACS Chem Biol. 2010 May 21;5(5):477–487. doi: 10.1021/cb900282e

Table 2.

Effects of osmolytes on proteins investigated in this study

	Protein only	+Betaine	ΔT_m	+Glycerol	ΔT_m	+4-PBA^a	ΔT_m	+Proline	ΔT_m	+Sarcosine	ΔT_m	+Sucrose	ΔT_m	+TMAO	ΔT_m
MBP-OLF	52.7 ± 0.80	59.6 ± 0.8	6.9	56.0 ± 1.2	3.3	55.5 ± 0.5	2.8	57.2 ± 0.3	4.5	65.2 ± 0.6	12.5	64.3 ± 0.1	10.6	64.1 ± 0.7	11.4
MBP- OLF(D380A)	46.7 ± 0.5	50.8 ± 0.2	4.1	49.5 ± 0.2	2.8	47.2 ± 0.1	0.5	49.9 ± 0.2	3.2	58.3 ± 0.6	11.6	54.2 ± 0.7	7.5	55.6 ± 0.9	8.9
MBP- OLF(I477S)	41.9 ± 0.5	48.0 ± 0.6	6.1	45.1 ± 0.2	3.1	42.6 ± 0.1	0.7	45.3 ± 0.3	3.4	54.0 ± 0.4	12.0	50.7 ± 0.2	8.7	52.7 ± 0.8	10.7
MBP- OLF(I477N)	40.1 ± 0.8	45.1 ± 0.7	4.9	43.7 ± 0.4	3.5	41.4 ± 0.4	1.3	43.8 ± 0.9	3.7	52.5 ± 0.3	12.4	50.3 ± 0.9	10.2	50.1 ± 0.8	9.9
MBP- OLF(K423E)	40.5 ± 0.1	45.9 ± 0.6	5.4	42.6 ± 0.2	2.0	38.9 ± 0.4	−1.6	42.7 ± 0.7	2.1	48.6 ± 0.6	8.1	46.3 ± 0.5	5.7	48.8 ± 0.7^b	8.3
MBP	67.1 ± 0.3	71.3 ± 0.6	4.2	68.2 ± 0.4	1.1	66.2 ± 0.1	−0.9	69.8 ± 0.6	2.7	75.1 ± 1.0	8.0	72.6 ± 0.8	5.5	74.4 ± 0.1	7.3