Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers used in this study
| MYOC-OLF cloning | |||
|---|---|---|---|
| Plasmid Template | Target Vector | Primer Used | |
| pcDNA | pET-30XaLIC | Forward | 5′-GGTATTGAGGGTCGCTTGAAGGAGAGCCCATCTG |
| Reverse | 5′-AGAGGAGAGTTAGAGCCTTATCACATCTTGGAGAGCTTGATG | ||
| pET-30XaLIC | pMAL-c4x | Forward | 5′-CGCCGAGCTCTATTGAGGGTCGC |
| Reverse | 5′-GCCGAATTCAGGAGAGTTAGAGCCTTATCA | ||
| Mutagenesis | |||
| D380A | 5′-GCTACACGGACATTGCCTTGGCTGTGGATG | ||
| K423E | 5′-GGGAGACAAACATCCGTGAACAGTCAGTCGCCAATGCC | ||
| I477N | 5′-GTACAGCAGCATGAATGACTACAACCCCCTGG | ||
| I477S | 5′-GTACAGCAGCATGTCTGACTACAACCCCCTCG | ||
