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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Traffic. 2010 Feb 27;11(6):732–742. doi: 10.1111/j.1600-0854.2010.01054.x

Figure 2. Effect of modifying Arf1 on nucleotide dissociation.

Figure 2

The time dependence of GTPγS (A) or GDP (B) dissociation from Arf1, Arf1-HA, Arf1-GFP or GST-Arf1 was determined as described in “Materials and Methods”. The data are expressed as a fraction of radiolabeled nucleotide bound to Arf at the time the reaction was initiated and were fit to single exponential decay equation, except GTPγS dissociation from Arf1-HA which was fit to a double exponential curve. The derived values for the dissociation rate are presented in Table 2. Data shown are one representative of three or four experiments.