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. Author manuscript; available in PMC: 2011 Jun 1.
Published in final edited form as: Traffic. 2010 Feb 27;11(6):732–742. doi: 10.1111/j.1600-0854.2010.01054.x

Figure 3. Effect of modifying Arf1 on ARNO1 catalyzed nucleotide exchange.

Figure 3

His6-ARNO1 was titrated into a GTPγS binding reaction as described in “Materials and Methods”. The data were fit to a hyperbola to estimate the amount of ARNO required to reach 50% of the maximal observed exchange in 5 min (C50), which is reported in Table 3. Data shown are representative of four experiments.