Table 3. Effect of modifications of Arf1 on ARNO1 catalyzed nucleotide exchange.

His₆-ARNO1 was titrated into a GTPγS binding reaction as described in “Materials and Methods”. The amount of ARNO1 required for 50% maximal exchange at 3 min is reported as the C50. The data are the mean ± S.E. of three experiments.

	Arf1	Arf1-HA	Arf1-GFP	GST-Arf1
C50 (μM)	0.037 ± 0.007	0.11 ± 0.03*	1.75 ± 0.05**	NE#

^*p<0.05 compared to wild type.

^**p<0.01 compared to wild type.

^#No effect