Skip to main content
. Author manuscript; available in PMC: 2010 Nov 20.
Published in final edited form as: Oncogene. 2010 Mar 8;29(20):2916–2926. doi: 10.1038/onc.2010.62

FIG 3.

FIG 3

Impact of manipulation of NOTCH/HES1 signaling on DTX1 expression. (A) PCR (left panel) and western blot (right panel) analysis of HES1, DTX1 and actin expression in OS187 cells overexpressing ICN1, dnMAM or HES1 compared to vector control. (B) Quantitative PCR analysis of HES1 and DTX1 in the same cells at 3A. Analysis was performed as in figure 1C. (C) COL cells, which have high endogenous DTX1, were manipulated and analyzed as in 3A. Left panel: PCR analysis. Right panel: western blot analysis. (D) Histograms represent matrigel invasion of the COL cells from 3C (*, p<0.05). Assay and analysis were performed as in figure 1E.

HHS Vulnerability Disclosure