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. Author manuscript; available in PMC: 2010 Nov 20.
Published in final edited form as: Oncogene. 2010 Mar 8;29(20):2916–2926. doi: 10.1038/onc.2010.62

FIG 6.

FIG 6

HES1 suppression increases DTX1 promoter activity through the N-box motif. (A) Immunoblot (IB) shows the impact of shRNA to HES1 or of dnHES1 on DTX1 and HES1 expression in OS187. Left panel: V indicates vector control; 5, 6, 7 and 8 indicate four distinct shRNAs specific for HES1. Actin serves as a loading control. Right panel: V indicates vector control; dhH indicates dominant negative HES1. Anti-HES1 antibody can detect both HES1 and dnHES1. (B) Wild type (WT) or N-box mutated (mutN) DTX1 promoter reporter construct (Renilla luciferase) was cotransfected with a firefly luciferase control into OS187 cells stably expressing empty vector, shHES1 or dnHES1. Relative luciferase activity was determined after 48-hr incubation. (*, p<0.05. **, p<0.005). (C) Histograms represent matrigel invasion of OS187 cells stably expressing empty vector or HES1-specific shRNA (clone 5 and 6). Analysis was performed as in figure 1E (*, p<0.05).

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