Fig. 1.

FISH analysis of clones from CFA10. (A) The chromosome location of five differentially labeled clones from the previously reported 10 Mb resolution dog BAC set (Thomas et al., 2007), starting with the most centromeric clone. The text color indicates the fluorochrome with which each clone was labeled for FISH analysis, and the Mb position on CFA10 is shown after the corresponding BAC address. To the left is the CFA10 ideogram, and to the right, three examples of these five probes hybridized to CFA10 at increasingly later stages of metaphase. Accurate assignment of clones at 10 Mb resolution is clearly possible in early metaphase, and probe order is easily ascertained. (B) Five probes at intervals of approximately 1 Mb, starting from the second clone in the previous 10 Mb set (326H08). In metaphase (Bi) these five probes at 1 Mb intervals can be accurately assigned to a chromosome band, but their relative order cannot be readily resolved due to extensive chromosome contraction. Accurate ordering is thus reliant on interphase mapping (Bii).