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. 2008 Dec;122(2):110–121. doi: 10.1159/000163088

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Copyright © 2008 by S. Karger AG, Basel

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Fig. 2 — Use of FISH analysis to resolve mapping anomalies. Clone 182G07 (CFA1; 97.5 Mb) mapped by FISH analysis to a site significantly more proximal to that suggested by the genome assembly (A). Co-hybridization of this BAC with two clones from this more proximal region of CFA1 resolved the location of 182G07 to approximately CFA1; 30 Mb (B, left image), whilst the gap left by this clone was successfully filled by clone 160A04 (CFA1; 97.2 Mb), selected from the assembly (B, right image). Using the same strategy, the location of clone 307O16 (CFA6; 61.9 Mb) was revised to a more proximal location (CFA6; 45 Mb, data not shown) and the consequent gap was filled successfully by clone 502O13 (CFA6; 62.0 Mb).